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MethodsNovel sensing solutions based on nanomaterials are arising as strategic tools able to surpass traditional sensor limits. Among these, Si nanowires (Si NWs), realized with cost-effective industrially compatible metal-assisted chemical etching, are perfect candidates for sEV detection.ResultsIn this paper, the realization of a selective sensor able to isolate, concentrate and quantify specific vesicle populations, from minimal volumes of biofluid, is presented. In particular, this Si NW platform has a detection limit of about 2× 10 5 sEVs/mL and was tested with follicular fluid and blastocoel samples. Moreover, the possibility to detach the selectively isolated sEVs allowing further analyses with other approaches was demonstrated by SEM analysis and several PCRs performed on the RNA content of the detached sEVs.DiscussionThis platform overcomes the limit of detection of traditional methods and, most importantly …
Dove Press
Publication date: 
1 Jan 2021

Antonio Alessio Leonardi, Rosalia Battaglia, Dario Morganti, Maria Josè Lo Faro, Barbara Fazio, Chiara De Pascali, Luca Francioso, Gerardo Palazzo, Antonia Mallardi, Michele Purrello, Francesco Priolo, Paolo Musumeci, Cinzia Di Pietro, Alessia Irrera

Biblio References: 
Volume: 16 Pages: 5153
International Journal of Nanomedicine