A single-shot damage induction to plasmid DNA was demonstrated by applying X-rays emitted by a laser-produced plasma. Yields of single-strand breaks and double-strand breaks were determined as a function of energy fluence which was adjusted by varying the distance of the exposed sample from the X-ray source and by thickness of Al filters attenuating X-rays. As an intense source of X-ray radiation was employed a double-stream gas puff target irradiated by sub-kJ, near-infrared (NIR) focused laser pulses at the PALS facility (Prague Asterix Laser System) to produce high-energy pulses of soft X-rays from hot, dense Xe plasma. The double-puff arrangement ensures high gas density and conversion efficiency from NIR to X-rays approaching that typical for solid targets. In addition, its major advantage over solid targets is that it is debris free and has substantially suppressed charged particle emission.
10 Sep 2008
Volume: 2008 Issue: 1 Pages: 14-17
1st Workshop-Plasmi, Sorgenti, Biofisica ed Applicazioni