This work presents the realization of a Si-based miniaturised device for liquid chromatography (LC), targeted for agrofood applications and in particular for wine quality monitoring. The main modules of the system are: (1) a Si-based separation column, functionalized by n-octyltriethoxysilane (C8-TEOS), with inlet/outlet for fluidic connections; (2) a three-microelectrode voltammetric sensor. The system has been properly packaged and the capability of the sensing module to detect acetic acid concentration changes has been verified.