1,8‐diazabicyclo[5.4.0]undec‐7‐ene (DBU) imprinted polymer was prepared as microspheres by precipitation polymerization method to obtain molecular recognition systems based on the noncovalent interactions between DBU template, methacrylic acid (MAA), and ethylene .glycol dimethacrylate (EDMA) in acetonitrile. 1H NMR analysis of DBU/MAA mixture has been performed and hydrogen bonding interactions have been established. Microspheres have been characterized by FTIR studies with evidence of DBU linkage in polymer particles and by Scanning Electron Microscopy (SEM) to study their morphological properties. How pH values affect the binding capacity of imprinted polymer during the binding stage has been also discussed and results suggest that imprinted poly‐(MAA‐EDMA) behavior is related to the influence of DBU basicity during rebinding processes and the optimum pH value for binding …