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Surface plasmon resonance imaging (SPRI) studies, performed on a specially designed system exploiting the Kretschmann configuration, have been carried out to develop a DNA sensor for the detection of gene mutations accounting for the analysis of a fungin species which can proliferate especially in cereals, producing toxic compounds such as mycotoxins. The SPRI system has been used in order to study the hybridization process of ssDNA carbonarius probes immobilized onto a bio-functionalized Au surface in order to detect in real time the mutations in a DNA fragment. The SPRI system is a good choice for real-time monitoring of hybridization dynamics on an array of immobilized oligonucleotide probes because of the high sensitivity in characterization of ultra-thin films adsorbed onto gold or other noble metal surfaces. Using this technique, local changes in the reflectivity of a thin metal film describe the …
IOP Publishing
Publication date: 
13 May 2008

MG Manera, Roberto Rella, Jolanda Spadavecchia, Julien Moreau, Michael Canva

Biblio References: 
Volume: 10 Issue: 6 Pages: 064018
Journal of Optics A: Pure and Applied Optics