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Protein-based microfibers are biomaterials of paramount importance in materials science, nanotechnology, and medicine. Here we describe the spontaneous in situ formation and secretion of nanostructured protein microfibers in 2D and 3D cell cultures of 3T3 fibroblasts and B104 neuroblastoma cells upon treatment with a micromolar solution of either unmodified terthiophene or terthiophene modified by mono-oxygenation (thiophene → thiophene S-oxide) or dioxygenation (thiophene → thiophene S,S-dioxide) of the inner ring. We demonstrate via metabolic cytotoxicity tests that modification to the S-oxide leads to a severe drop in cell viability. By contrast, unmodified terthiophene and the respective S,S-dioxide cause no harm to the cells and lead to the formation and secretion of fluorescent and electroactive protein–fluorophore coassembled microfibers with a large aspect ratio, a micrometer-sized length and …
American Chemical Society
Publication date: 
6 Apr 2022

Ilaria Elena Palamà, Gabriele Maiorano, Francesca Di Maria, Mattia Zangoli, Andrea Candini, Alberto Zanelli, Stefania D’Amone, Eduardo Fabiano, Giuseppe Gigli, Giovanna Barbarella

Biblio References: 
Volume: 7 Issue: 15 Pages: 12624-12636
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