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The adsorption processes of oligonucleotides immobilised onto suitable photolithographic patterned gold substrates have been investigated in aqueous buffer solution by using a home made surface plasmon resonance (SPR) imaging equipment. A rapid self-assembled method for the construction of DNA chips to be used in SPR imaging experiments have been followed. The immobilised DNA molecules (probes) adopted in our SPR experiments anchored to a gold surface via thiol group were 5′thiol-modified containing a (CH2)15 tail. The hybridisation processes taking place with its complementary sequence have been observed and characterized by monitoring phenomena by a SPR imaging system. The two analysed oligonucleotides (probes and target) are of interest in plant gene biotechnological application and differing for the presence at the 5′-end of a poly T16 spacer. Dynamic investigation of smallest …
Publication date: 
15 Dec 2005

J Spadavecchia, MG Manera, F Quaranta, P Siciliano, R Rella

Biblio References: 
Volume: 21 Issue: 6 Pages: 894-900
Biosensors and Bioelectronics